Top 80+ Solved Sterilisation, Culture Media and Pure Culture Techniques MCQ Questions Answer
Q. Cultures are prepared by penetrating the inoculation loop with suspension into the medium, they are
a. Stock culture
b. Stabcultures
c. Sub-cultures
d. None of these
Q. The principle involved in the streak platemethod is
a. Separation
b. Streaking
c. Isolation
d. Dilution
Q. Culture media for fungi are
a. Potato dextrose agar (PDA)
b. Sabouraud’s agar
c. Czapekdox agar
d. All of the above
Q. Spores of actinomycetes are verysensitive, killed at room temperature of
a. 52oC for 30 min.
b. 65oC for 30 min.
c. 70oC for 30 min.
d. 43oC for 30 min.
Q. The term that is used for the bacteria which can withstand pasteurization but does not grow at higher temperatures
a. Thermophiles
b. Extreme thermophiles
c. Thermoduric
d. Facultative thermophiles
Q. A common laboratory method ofcultivating anaerobic micro-organisms is
a. Gas pack system
b. Brewer jar system
c. Pyrogallic acid over the cotton
d. None of these
Q. The micro-organisms grow at highsalinity are
a. Osmophile
b. Halophiles
c. Both a and b
d. None of these
Q. Non-lactose fermenting colonies seen onMac Conkey’s medium are
a. Salmonella typhi
b. Escherichia coli
c. Klebsiella pneumoniae
d. Shigella shigae
Q. Wilson and Blair medium is used forisolation of
a. Staphylococci
b. Salmonella typhosa
c. Vibrio cholerae
d. Shigella shigae
Q. Laboratory diagnosis of enteric fever isbased on
a. Blood culture
b. Urine and stool culture
c. Widal test
d. All of the above
Q. Which of the following are gas producingSalmonella?
a. S.typhi
b. S.enteritidis
c. S.cholerasuis
d. S.typhimurium
Q. Kauffmann white scheme is used to detect
a. Salmonella spp.
b. Shigella spp.
c. E.coli
d. None of these
Q. On Mac Conkey’s medium Esch. Coli forms
a. Colorless colonies
b. Greenish pigmentation
c. Pink coloured colonies
d. Medusa head appearance