Top 80+ Solved Biotechnology Principles and Processes MCQ Questions Answer
Q. These are important set of enzymes used in biotechnology Match them with exact roleP Taq DNa Polymerase (i) cutting single stranded part of DNAQ S I nuclease (ii) LigaseR Restriction endo nuclease (iii) Thermostable enzymeS mole cular glue (iv) cutting pallindromic sequences(v) union of pallindromic sequencesP Q R S
a. (iii) (iv) (i) (ii)
b. (iii) (v) (iv) (ii)
c. (iv) (i) (v) (ii)
d. (iii) (iv) (i) (ii)
Q. For transformation, micro particles coated with DNA are bombarded with gene gun made up of.
a. Platinum or Zinc
b. Silicon or Platinum
c. Gold or tungsten
d. Silver or Platinum
Q. PCR and Restriction fragment lenth Polymorphism are the methods for. (AIPMT-2012)
a. genetic transtormation
b. DNA Sequencing
c. DNA finger printing
d. Study of enzymes
Q. The linking of antibiotic resistance gene with the plasmid Vector became possible with
a. DNA ligase
b. Exonuclease
c. Endo nuclease
d. DNA Polymerase
Q. Gel electrophoresis is used for--
a. Isolation of DNA molecule
b. Cutting of DNA in to fragments
c. Separation of DNA fragments according to their size
d. Construction of recombinant DNA by joining with cloning Vector
Q. Gentic engineering has been sucessfully used for producing ___ (CBSE-2010)
a. trangenic models for studying new treatments for Certain cardiac diseases.
b. transgenic Cow - Rosie which produces high fat milk for making ghee.
c. animals linke bulies for farm work as they have super power.
d. transgenic mice for testing safety of polio Vaccine before use in humans.
Q. Match the following and choose the correct combination from the option given ... (Karnatak PMT-2005)Column I Column II(a) Escherichia coli 1 - nif gene(b) Rhizobium meliloti 2 - digestion of hydrocarbons of crude oil(c) Bacilius thuringiensis 3 - human insulin production(d) Pseudomonas putida 4 - Biocontrol of fungal disease5 - biodegradable insecticide
a. A = 3, B = 1, C = 5, D = 4
b. A = 1, B = 2, C = 3, D = 4,
c. A = 2, B = 1, C = 3, D = 4
d. A = 4, B = 3, C = 1, D = 2
Q. Find the incorrrect statement
a. Gene therapy is a genetic engineering technique used to treat disease at molecular level by replacing defective genes with normal genes.
b. Calcitonin is a medically useful recombinant product in the treatment of intetility
c. Bt toxin is a Biodegradable insecticide obtained from bacillis thuringiensis
d. Trichoderma sp. is a biocontrol agent for fungal diseases of plants
Q. Production of a human protein in bacteria genetic engineering is possible because
a. bacterial cell can carry out the RNA splicing reactions
b. the human chromosome can replicate in bacterial cell
c. the mechanism of gene regulation is identical in human and bacteria
d. The genetic code is universal
Q. The basis of DNA finger printing is
a. The double helix
b. Errors in base sequence
c. Poly morphism in sequence
d. DNA replication
Q. A genetically engineered microorganism used successfully in biomediation of oil spillg is species of (CBSE-2007)
a. Trichoderma
b. Xamthomonas
c. Bacillus
d. Pseudomonas
Q. What is the function of Restriction endonuclease ? (AIPMT -2006)
a. Restricts the synthesis of DNA inside the nucleus
b. Synthesizes DNA
c. Cuts DNA molecule randomly
d. cuts DNA molecule at specific sites
Q. The nuclease enzyme which begins its attack from Free end of a polynucleotide is
a. Exonuclease
b. Kinase
c. Polymerase
d. Endonuclease